Augmentation of retinoic acid‐induced granulocytic differentiation in HL‐60 leukemia cells by serine/threonine protein phosphatase inhibitors

FEBS Letters

Deguchi, Katsumi⁴  Nagao, Minako³  Kuno, Takayoshi²  Shirakawa, Shigeru⁴  Itoh, Masaaki¹  Kobayashi, Kazuhiko⁴  Morita, Koichi⁴  Nakano, Takeshi¹  Nishikawa, Masakatsu⁴  Shima, Hiroshi³  Tanaka, Chikako² 

[1] First Departments of Internal Medicine, Mie University School of Medicine, Tsu, Carcinogenesis Division, Tokyo Japan;Department of Pharmacology, Kobe University School of Medicine, Kobe, Japan;National Cancer Research Institute, Tokyo Japan;Second Departments of Internal Medicine, Mie University School of Medicine, Tsu, Carcinogenesis Division, Tokyo Japan

关键词: HL-60 cell;    Differentiation;    Phosphatase;    Okadaic Acid;    Calycutin-A;   

DOI  :  10.1016/0014-5793(92)81501-C

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

To evaluate the involvement of protein phosphatases (PP) in differentiation of human myclogenous leukemia HL-60 cells, we made use of potent inhibitors of PP1 and PP2A, calyculin-A (CAL-A) and okadaic acid (OKA). CAL-A and OKA could augment all-trans retinoic acid (ATRA)-induced granulocytic differentiation, whereas the differentiation toward macrophage lineage by 12-o-tetradecanoylphorbol acetate (TPA) was unchanged in the presence of CAL-A. CAL-A augmented the phosphorylation or 18K, 23K and 30K proteins induced by ATRA. The PP1 and PP2A were identified and were present mainly in the cytosol of HL-60 cells. These results suggest that either PP1 or PP2A or both may be involved in regulating granulocytic differentiation or HL-60 cells.

【 授权许可】

Unknown