The murine NPY‐1 receptor gene Structure and delineation of tissue‐specific expression

FEBS Letters

Oberto, Alessandra¹  Genazzani, Enrico¹  Sprengel, Rolf²  Eva, Carola¹ 

[1] Istituto di Farmacologia e Terapia Sperimentale, University of Torino, 10125 Torino, Italy;Zentrum für Molekulare Biologie, University of Heidelberg, D-6900 Heidelberg, Germany

关键词: G protein receptor;    Neuropeptide Y;    Neuropeptide receptor;    Gene structure;    Gene expression;    NPY;    neuropeptide Y;    bp;    base pair;    kb;    kilobases;    PIPES;    piperazine-N;    N′-bis[2-ethane sulphonic acid];   

DOI  :  10.1016/0014-5793(92)81490-D

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The murine gene for the NPY-1 receptor subtype for neuropeptide Y was characterized by DNA sequencing and expression studies. It comprises three exons with a 6,400 bp 5′-untranslated and a 80 bp internal intronic sequence. The 5′-flanking region of this gene lacks TATA or CCAAT consensus sequences in the proximity to the multiple transcription initiation sites. A 1,300 bp genomic fragment of the 5′-flanking region drives the expression of the lacZ reporter gene in NG108-15 cells and primary cultured neurons but not in glial and human embryonic kidney cells. In addition, it contains consensus sequences for various transcription factors including cAMP- and glucocorticoid-responsive elements.

【 授权许可】

Unknown