Investigation of the neighbour relationships between photosystem II polypeptides in the two types of isolated reaction centres (D1/D2/cytb 559 and CP47/D1/D2/cyt b 559 complexes)

FEBS Letters

Moskalenko, Andrey A.¹  Barbato, Roberto²  Giacometti, Giorgio M.² 

[1] Institute of Soil Science and Photosynthesis RAS, 142292 Pushchino, Russian Federation;Dipartimento di Biologia, Università di Padova, via Trieste 75, I-35121 Padova, Italy

关键词: Photosystem II;    Reaction center;    Crosslinker;    Nearest neighbour;    Photobleaching;    Photocrosslinking;    PS;    photosystem;    RC;    reaction center;    CP;    chlorophyll protein;    cyt;    cytochrome;    Chl;    chlorophyll;    Pheo;    pheophytin;    EDC;    1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride;    NHS;    N-hydroxysulfosuccinimide;    DST;    disuccinimidyl tartarate;    DSP;    dithiobis(succinimidylpropionate);    EGS;    ethylenglycolbis(succinimidyl succinate);    DDIMB;    2;    5-dibromo-3-methyl-6-isopropyl-p-benzoquinone;   

DOI  :  10.1016/0014-5793(92)81487-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The nearest neighbour relationships within the D1/D2/cyt b ₅₅₉ complex (PSIIRC) and the CP47/D1/D2/cyt b ₅₅₉ complex (RC-CP47) were investgated by using different length bifunctional crosslinking agents. The crosslinking products were identified by immunoblotting with polyclonal antibodies and by two-dimensional gel electrophoresis. Seven products (CP47/D2, D1/D2/α, D1/D2, D2/α, D1/α, α/αa, α/β) have been revealed in both complexes. The crosslinking of both complexes does not increase their photostability. The photocrosslinking products (D1/α and D2/α) appeared under illumination of complexes with light of high intensity.

【 授权许可】

Unknown