Receptor mediated mineralocorticoid action in alga cell mutants

FEBS Letters

Mirshahi, A.²  Mirshahi, M.²  Nato, A.³  Agarwal, M.K.¹ 

[1] Hormone Laboratory, Centre Universitaire des Cordeliers, Paris, France;INSERM U-86, Centre Universitaire des Cordeliers, Paris, France;CNRS URA 1128, Centre Universitaire des Cordeliers, Paris, France

关键词: Alga;    Chlamydomonas;    Mineralocorticoid;    Receptor;    Aldosterone;    Spirolactone;    RU 26752;   

DOI  :  10.1016/0014-5793(92)81479-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The multiplication of Chlamydomonas cells can be arrested by the spirolactone derivative RU 26752 and this is fully reversible by the natural hormone aldosterone. Continuous growth in the presence of RU 26752 led to the isolation of a population subsequently resistant to the action of mineralocortoid analogues, due possibly to the selection of mutant cells. Immunophotochemical evidence is provided for a 52 kDa protein that possesses functional steroid and DNA binding domains. Alga cells therefore appear to respond to steroid hormones in a manner similar to the mammalian systems, possibly via a receptor that may represent a pygmy ancestor of the latter day steroid receptor superfamily.

【 授权许可】

Unknown