‘All‐or‐none’ mechanism of the molten globule unfolding

FEBS Letters

Uversky, V.N.¹  Ptitsyn, O.B.¹  Pain, R.H.²  Semisotnov, G.V.¹ 

[1] Institute of Protein Research, Russian Academy of Sciences, 142292 Pushchino, Moscow Region, Russian Federation;Department of Biochemistry and Genetics, University of Newcastle-upon-Tyne, Newcastle-upon-Tyne NE2 4HH, UK

关键词: Protein folding;    Molten globule;    Size exclusion chromatography;    Carbonic anhydrase B;    β-Lactamase;    N;    native state;    MG;    molten globule state;    U;    unfolded state of protein molecules;    BCAB;    bovine carbonic anhydrase D;    Gdm-HCl;    guanidinium hydrochloride;    ANS;    1-anilino-naphthalene-8-sulphonate;    CD;    circular dichroism;    UV;    ultraviolet;   

DOI  :  10.1016/0014-5793(92)81468-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The Gdm-HCl-induced unfolding of bovine carbonic anhydrase B andS. aureus β-lactamase was studied at 4°C by a variety of methods. With the use of FPLC it has been shown that within the transition from the molten globule to the unfolded state the distribution function of molecular dimensions is bimodal. This means that equilibrium intermediates between the molten globule and the unfolded states are absent, i.e. the molten globule unfolding follows the ‘all-or-none’ mechanism.

【 授权许可】

Unknown