Interaction of human myeloperoxidase with nitrite

FEBS Letters

Cooper, Chris E.¹  Odell, Eddy² 

[1] Metals in Biology and Medicine Centre, Division of Life Sciences, King's College London, Campden Hill Road, London W8 7AH, UK;Dept. Oral Medicine and Pathology, UMDS, Guy's Hospital, London Bridge, London SE1 9RT, UK

关键词: Myeloperoxidase;    Isoenzyme;    Neutrophil;    Electron paramagnetic resonance;    Nitrite;    Nitric oxide;   

DOI  :  10.1016/0014-5793(92)81461-T

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

EPR (electron paramagnetic resonance) and optical spectroscopy show that human neutrophil myeloperoxidase is converted from ferric high-spin to low-spin by the addition of nitrite. The Soret peak shifts from 429 to 447 nm and new peaks appear in the visible region at 573 and 627 nm; the EPR g-values change from 6.84, 5.02, 1.95 to 2.55, 2.31, 1.82. Small differences are seen in the EPR (but, not optical) spectra of myeloperoxidase isoenzyme I compared to isoenzymes II and III. The reaction with nitrite is detectable by EPR in intact neutrophils and is thus a possible in vivo monitor of NO/nitrite production by these cells.

【 授权许可】

Unknown