Inhibition of calcineurin by cyclosporin A‐cyclophilin requires calcineurin B

FEBS Letters

Haddy, Alice¹  Rusnak, Frank¹  Swanson, Selene K.-H.¹  Born, Timothy L.¹ 

[1] Section of Hematology Research, Mayo Foundation and Clinic, Rochester, MN 55905, USA

关键词: Calcineurin;    Cyclosporin A;    Cyclophilin;    Immunosuppression;    CaM;    calmodulin;    CN;    calcineurin;    CNA;    calcineurin A;    CNB;    calcineurin B;    CsA;    cyclosporin A;    CyP;    cyclophilin;    hCyPA;    recombinant human cyclophilin A;    FKBP;    FK506 binding protein;    hCyPB;    recombinant human cyclophilin B;    PP;    protein phosphatase;    rCNA;    recombinant calcineurin A;   

DOI  :  10.1016/0014-5793(92)81456-V

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The interaction of the immunosuppressive complex cyclosporin A-cyclophilin (CsA-CyP) with the Ca²⁺/calmodulin-dependent protein phosphatase calcineurin is investigated using a recombinant form of the A subunit of calcineurin (rCNA). Only in the presence of purified calcineurin B (CNB) does rCNA show the response of native calcineurin, i.e. 50% inhibition of rCNA phosphatase activity at 6 nM human cyclophilin B and 0.6 μM human cyclophilin A using [³²P]casein as substrate, yet stimulation of activity with p-nitrophenyl phosphate as substrate. This study demonstrates that the B subunit is necessary to confer sensitivity of calcineurin to CsA-CyP.

【 授权许可】

Unknown