Interaction of α subunit of GTP‐binding protein Go with a 20‐kDa Triton‐insoluble membrane protein in bovine brain

FEBS Letters

Takahashi, Satoru¹  Negishi, Manabu¹  Ichikawa, Atsushi¹  Shiozaki, Tateki¹ 

[1] Department of Physiological Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University, Kyoto 606, Japan

关键词: GTP-binding protein;    Triton-insoluble fraction;    Bovine brain;   

DOI  :  10.1016/0014-5793(92)81453-S

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Heterotrimeric Go bound to the membranes of bovine brain, but Goα remained bound to the membranes even after activation with GTPγS. Furthermore, Goα bound to a Triton X-100-insoluble fraction of the membranes in a saturable manner. However, the 37-kDa Goα eliminated by trypsin at the amino-terminus could not bind to the fraction. Using a blot overlay approach of the Triton-insoluble fraction, only a 20-kDa protein was identified that interacts with Goα. These results indicate that Goα binds to a 20-kDa Triton-insoluble protein in the bovine brain membranes.

【 授权许可】

Unknown