Ganglio‐N‐tetraosylceramide (GA1) of bovine and human brain Molecular characterization and presence in myelin

FEBS Letters

van Halbeek, H.²  Hogan, E.L.¹  Dasgupta, S.¹ 

[1] Department of Neurology, Medical University of South Carolina, Charleston, SC 29425, USA;Complex Carbohydrate Research Center, The University of Georgia, Athens, GA 30602, USA

关键词: Bovine brain;    Myclin;    Neutral glycosphingolipids;    NMR;    TLC;    thin-layer chromatography;    Ngsl;    neutral glycosphingolipid;    GM1;    II3NeuAcGgOSe4Cer;    GM1b;    IV-NeuAcGg-Ose4Cer;    GA1 or GgOse4Cer;    III3GalGg3Cer;    Gb3Cer;    Galα1→4 Galβ1→4GlcCer;    Gg;    Cer;    Gal1NAcβ1→-4Galβ1→4GlcCer;    LacCer;    Galβ→4GlcCer;    nLc4Cer;    Galβ→3GlcNAcββ4Galβl→4GlcCer;    nLc5Cer;    IV3GalnLc4Cer. Gangliosides are designated following Svennerholm;    L. (1963) J. Neurochem. 10;    613–623;   

DOI  :  10.1016/0014-5793(92)81234-D

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

During our studies of bovine brain neutral glycosphingolipids (Ngsl's), we have purified a compound that co-migrates on thin-layer chromatogram with standard GA1 (purified by acid hydrolysis of GM1) and close to penta- (nLc₅Cer) glycosylceramide from bovine erythrocytes. The structure of the purified Ngsl from brain has been established by permethylation and by stepwise exoglycosidase hydrolysis. 600 MHz ¹H NMR spectroscopy of the oligosaccharide obtained from the Ngsl after endoglycoceramidase hydrolysis confirms the structure as ganglio-N-tetraosylceramide (GgOse₄Cer or GA1) as Ga1β1→3Ga1NAcβ1-4Galβ1→4G1cβ1→1Cer. We have identified GA1 in bovine, rat and human brain and myelin by TLC-immunostaining with monospecific anti-GA1 antiserum.

【 授权许可】

Unknown