Human peripheral blood lymphocytes express D5 dopamine receptor gene and transcribe the two pseudogenes

FEBS Letters

Yanagihara, Takehiko¹  Ueno, Satoshi¹  Saeki, Yoshinaga¹  Nagai, Yoshitaka¹  Takahashi, Nobuyuki¹ 

[1] Department of Neurology, Osaka University Medical School, 1-1-50 Fukushima, Fukushima-ku, Osaka 553, Japan

关键词: Dopamine receptor;    Lymphocyte;    Reverse transcription;    Polymerase chain reaction;    RT;    reverse transcription;    PCR;    polymerase chain reaction;    DR;    dopamine receptor gene;    bp;    base pairs;   

DOI  :  10.1016/0014-5793(92)81452-R

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Sequential reverse transcription and polymerase chain reaction (RT-PCR) of the mRNA were used to investigate the expression of dopamine receptors in human peripheral blood lymphocytes. The RT-PCR products contained three types of sequences, each corresponding to those of the D5 dopamine receptor gene and the two related pseudogenes. The lymphocyte cDNA library also contained the clones encoding parts of the three genes. Binding profiles of dopaminergic ligands to the lymphocytes were similar to those for the native neuronal membranes.

【 授权许可】

Unknown