FEBS Letters | |
Glycation of crystalline in lenses from aging and diabetic individuals | |
van Boekel, M.A.M.1  Hoenders, H.J.1  | |
[1] Department of Biochemistry, University of Nijmegen, Nijmegen, The Netherlands | |
关键词: Glycation; Diabetes; Aging; Crystallin; Cataract; Lens; HM; high molecular weight material; HPGPC; high pressure gel permeation chromatography; BCA; bicinchoninic acid; WS; water soluble; EDTA; ethylene diamino tetra-acetate; | |
DOI : 10.1016/0014-5793(92)81446-S | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Water-soluble crystallins were obtained from clear human lenses of different age(4–81-year-olds) and lenses of individuals showing senile and diabetic cataracts. Levels of early glycation products were high in the high molecular weight material (HM) and the α-crystallin fractions, compared with β and γ-crystallins. This difference becomes more prominent upon aging. The content of total early glycation products in HM and α-crystallin increases clearly with age, whereas levels remain relatively constant in the β- and α-crystallins. There is an elevation of early products in cataractous lenses from diabetic individuals compared with those suffering from senile cataract. Specific non-tryptophan fluorescence (excitation/emission wavelengths 370/440 nm), used as an indicator for late glycation products, increased dramatically with age and was 2-fold higher in the diabetic subjects. Levels of fluorescence decreased in the order HM > α- > b > γ-crystallins. The results suggest an increase in glycation rate in α-crystallin as a result of aging and diabetes, while the rate of glycation of β- and γ-crystallins remains almost constant.
【 授权许可】
Unknown
【 预 览 】
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