Inhibition by spermine of the inner membrane permeability transition of isolated rat heart mitochondria

FEBS Letters

Lapidus, Rena G.¹  Sokolove, Patricia M.¹ 

[1] The Department of pharmacology and Experimental Therapeutics, University of Maryland School of Medicine, Baltimore, MD 21201, USA

关键词: Spermine;    Mitochondria;    Permeability transition;    Ca2+;    Swelling;    BSA;    bovine serum albumin;    fraction V;    CAT;    carboxyatractylate;    CsA;    cyclosporin A;    DMSO;    dimethyl sulfoxide;    EGTA;    ethylene glycol bis(β-aminoethyl ether)-N;    N;    N′;    N′-tetracetic acid;    HEPES;    4-(2-hydroxyethyl)-1-piperazine-ethanesulfonic acid;    i.m.;    inner membrane;    MOPS;    3-(N-morpholino)propane-sulfonic acid;    PhAsO;    phenylarsine oxide;    Pi;    inorganic phosphate;    t-BH;    t-butyl hydroperoxide;   

DOI  :  10.1016/0014-5793(92)81217-A

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect of spermine on the permeability transition of the inner mitochondrial membrane of isolated rat heart mitochondria was evaluated. The permeability transition was triggered using a series of agents (t-butyl hydroperoxide, phenylarsine oxide, carboxyatractylate, and elevated Ca²⁺ and inorganic phosphate concentrations), and was monitored via Ca²⁺-release, mitochondrial swelling and pyridine nucleotide oxidation. By all three criteria, spermine inhibited the transition. A C₅₀ of 0.38 ± 0.06 (SD) mM was measured for inhibition.

【 授权许可】

Unknown