Spleen protein tyrosine kinases TPK‐IIB and CSK display different immunoreactivity and opposite specificities toward c‐src‐derived peptides

FEBS Letters

Cesaro, Luca¹  Fagard, Remi²  Brunati, Anna Maria¹  Benarous, Richard²  Fischer, Siegmund²  Bougeret, Cecile²  Donella-Deana, Arianna¹  Pinna, Lorenzo A.¹  Marin, Oriano¹  Allee, Guillaume² 

[1] Dipartimento di Chimica Biologica and Centro per lo Studio della Fisiologia Mitocondriale del Consiglio Nazionale delle Ricerche, Università di Padova, Padova, Italy;Unité 332, Institut National de la Santé et de la Recherche Médicale, Paris, France

关键词: Protein tyrosine kinase;    pp6Ocsrcregulation;    Phosphotyrosine;    Peptide;    Spleen;   

DOI  :  10.1016/0014-5793(92)81212-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Polyclonal antibodies have been raised against two synthetic peptides reproducing the 48–64 and 353–369 sequences of CSK, a protein tyrosine kinase implicated in the down-regulation of src-related protein kinases. Both antibodies specifically recognize recombinant CSK and a CSK-related 49 kDa protein tyrosine kinase present in spleen but they do not cross-react with purified TPK-IIB, a spleen protein tyrosine kinase sharing with CSK catalytic activity toward src kinases and incapability to autophosphorylate. CSK and TPK-IIB once resolved from each other by heparin-Sepharose affinity chromatography, display opposite specificities toward synthetic peptides reproducing the sequences around the main phosphoacceptor residues of pp6O^csrc , namely Tyr-416 and Tyr-527. These data support the view that TPK-IIB and CSK may exert opposite effects on the activity of src-related protein tyrosine kinases.

【 授权许可】

Unknown