Phosphorylation of tyrosine prevents dityrosine formation in vitro

FEBS Letters

Seelos, Christian²  Gmeiner, Bernhard¹ 

[1] Department of Medical Chemistry, University of Vienna, Waehringerstrasse 10, A-1090 Vienna, Austria;Department of Nutrition and Biological Sciences, University of Vienna, Waehringerstrasse 10, A-1090 Vienna, Austria

关键词: Tyrosine;    Dityrosine;    Phosphotyrosine;   

DOI  :  10.1016/0014-5793(89)81130-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Treatment of L-tyrosine in a peroxidase/H₂O₂, system results in the formation of dityrosine. However, the phosphoester derivative of tyrosine, O-phospho-L-tyrosine, was unable to form dityrosine even in mixtures with free L-tyrosine. Dephosphorylation of O-phospho-L-tyrosine by alkaline phosphatase followed by horseradish peroxidase/H₂O₂, treatment resulted in the formation of dityrosine. Our in vitro results indicate that phosphorylation/dephosphorylation of L-tyrosine may regulate dityrosine formation, and is supposed to play an important role in protein-protein interactions, i.e. cross-linking.

【 授权许可】

Unknown