Glucose decreases respiratory control ratio in EL‐4 tumor cells

FEBS Letters

Gabai, Vladimir L.¹ 

[1] Institute of Medical Radiology, Russian Academy of Medical Sciences, Obninsk 249020, Russian Federation

关键词: Respiration;    Aerobic glycolysis;    Respiratory control;    Oxidative phosphorylation;    EL-4 ascites tumor cell;    CCCP;    carbonyl cyanide m-chlorophenylhydrazone;    DNP;    2;    4-dinitrophenol;    EAC;    Ehrlich ascites carcinoma;    HBSS;    Hank's balanced salt solution;    RCR;    respiratory control ratio.;   

DOI  :  10.1016/0014-5793(92)81427-N

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

EL-4 ascites thymoma cells are shown to have high aerobic glycolysis and decreased Pasteur effect. At the same time, glucose produces a much smaller inhibitory effect on cell respiration (Crabtree effect) than in Ehrlich ascites carcinoma (EAC) cells. In intact EL-4 cells, the respiratory control ratio (RCR) was found to be 6.2 with endogenous substrates and 8.0 with glutamine. Glucose decreased the RCR to 3.2, by stimulating, the state 4 respiration. In rat thymocytes and EAC cells, such an effect of glucose was absent (RCR of 7.0 and 7.2, respectively). It is suggested that in EL-4 tumor cells, the high aerobic glycolysis and small Crabtree effect may be due to glucose-induced ‘uncoupling’ of oxidation and phosphorylation.

【 授权许可】

Unknown