Osteoclasts express high levels of p60c‐src , preferentially on ruffled border membranes

FEBS Letters

Fukui, Yasuhisa³  Suda, Tatsuo¹  Kurokawa, Takahide²  Tanaka, Sakae¹  Udagawa, Nobuyuki¹  Sasaki, Takahisa¹  Takahashi, Naoyuki¹ 

[1] Departments of Biochemistry and Oral Anatomy, School of Dentistry, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo, 142, Japan;Department of Orthopedic Surgery, School of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113, Japan;Laboratory of Biochemistry, Department of Agricultural Chemistry, University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113, Japan

关键词: Osteoclast;    p60c-src;    Tyrosine kinase;    1α;    25-dihydroxyvitamin D3;    Osteopetrosis;   

DOI  :  10.1016/0014-5793(92)81190-W

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Expression of p60^c-src , the normal cellular counterpart of the transforming protein of Rous sarcoma virus (RSV), p60^c-src , was examined in mouse and rat authentic osteoclasts and mouse osteoclast-like multinucleated cells (MNCs) formed in vitro. In co-cultures of mouse osteoblastic cells and spleen cells, the expression of p60^c-src strikingly increased on day 5 in parallel with the appearance of MNCs in the presence of 1α,25-dihydroxyvitamin D₃ (1α,25(OH)₂D₃). Immunohistochemical examination confirmed the high level expression of p60^c-src in both mouse authentic osteoclasts and MNCs. Electron microscopic examination revealed that p60^c-src was primarily localized on ruffled border membranes and vacuoles, but not on the clear zone in rat authentic osteoclasts. These results suggest that p60 ^c-src is important in osteoclastic bone resorption.

【 授权许可】

Unknown