Characterisation of the expression and post‐translational processing of the preprotachykinin‐I gene and the regulated release of tachykinins by the RINm5F cell‐line

FEBS Letters

Göke, Rüdiger¹  Hartel, Robert²  Fehmann, Hans-Christoph¹  Lankat-Buttgereit, Brigitte¹  McGregor, Gerard P.²  Göke, Burkhard¹  Voigt, Karlheinz² 

[1] Department of Internal Medicine, Baldingerstr., University of Marburg W-3550 Marburg, Germany;Department of Physiology, Deutschhausstr. 2, University of Marburg, W-3550 Marburg, Germany

关键词: Preprotachykinin-I;    Tachykinin;    RINm5F cell;    Post-translational processing;    Regulated release;    PPT;    preprotachykinin;    SP;    substance P;    NKA;    neurokinin A;    NKB;    neurokinin B;    IR;    immunoreactivity;    RIA;    radioimmunoassay;    rpHPLC;    reversed-phase high-pressure liquid chromatography;    EDTA;    ethylene diaminetreta-acetic acid;    8-Br-cAMP;    8-bromo-cyclic adenosine monophosphate;    HEPES;    N-2-hydroxy-ethylpiperazine-N′-2-ethanesulfonic acid;   

DOI  :  10.1016/0014-5793(92)80932-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Normal transcription and postranslational processing of the preprotachykinin (PPT)-I gene and regulated release of substance P and neurokinin A by the rat pancreatic endocrine cell-line, RINm5F, has been demonstrated, using radioimmunoassays (RlAs), reversed-phase (rp)HPLC and Northern blot analysis. This is the first stable cell-line found to express the PPT-I gene and provides an opportunity for investigating PPT-I gene expression and tachykinin biosynthesis. RIN5mF cells are a model for the pancreatic beta-cell, which is not known to exhibit PPT-I gene expression which may, therefore, be a feature of the transformed state of these cells. These data may imply that the tachykinins are important in pancreatic islet embryogenesis.

【 授权许可】

Unknown