Comparative analysis of sodium‐dependent l‐glutamate transport of synaptosomal and astroglial membrane vesicles from mouse cortex

FEBS Letters

Danbolt, Niels C.²  Kanner, Baruch I.³  Rauen, Thomas¹  Jeserich, Gunnar¹ 

[1] Department of Animal Physiology, University of Osnabrück, W4500 Osnabrück, Germany;Department of Anatomy, Institute of Basic Med. Science, PO Box 1105, Blindern, N-0317 Oslo 3, Norway;Department of Biochemistry, Hadassah Medical School, The Hebrew University, PO Box 1172, Jerusalem, Israel

关键词: Astroglial membrane vesicle;    l-Glutamate transport;    Synaptosome;    Immunoblotting;    Immunocytochemistry;    Glutamate analogue;    CCCP;    carbonyl-cyanide-m-chlorophenylhydrazone;    Tris;    Tris(hydroxymethyl)-aminomethane;    l-trans-PDC;    l-transpyrrolidine-2;    4-dicarboxylate;   

DOI  :  10.1016/0014-5793(92)81401-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Uptake of [³H]l-glutamate into membrane vesicles prepared from either mouse cortical astrocyte cultures or synaptosomes was found to be an electrogenic sodium- and potassium-dependent transport process with saturable uptake kinetics. Pharmacological differences were revealed by using a variety of substrate analogues. l-trans-PDC inhibited the synaptosomal glutamate transport 2–4-fold stronger than the astroglial uptake. The substrate analogues dl-threo-β-hydroxy-aspartate, dl-aspartate-β-hydroxamate, l-aspartate and d-aspartate inhibited glutamate transport of astroglial and neuronal membrane vesicles in a distinctive manner, whereas d-glutamate, quisqualate and dihydrokainate had no effect in either case. Immunoblotting and immunocytochemical labeling with antibodies against the rat brain glutamate transporter revealed the selective reaction of a band at about 75 kDa mol. wt. and a specific pattern of astrocyte immunostaining.

【 授权许可】

Unknown