Pyruvate utilization by synaptosomes is independent of calcium

FEBS Letters

Kauppinen, Risto A.¹  Nicholls, David G.¹ 

[1] Neurosciences Research Group, Department of Psychiatry, Ninewells Medical School, University of Dundee, Dundee DD1 9SY, Scotland

关键词: Pyruvate;    Synaptosome;    Ca2+;    ΔΨp;    plasma membrane potential;    ΔΨm;    in situ mitochondrial membrane potential;    [Ca2+]c;    cytosolic free Ca2+ concentration;    [Ca2+]m;    mitochondrial free Ca2+ concentration;    PDHA;    PDHB;    pyruvate dehydrogenase A and B;   

DOI  :  10.1016/0014-5793(86)80484-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The significance of Ca²⁺ is assessed for the activation of pyruvate oxidation by intact nerve terminals (synaptosomes). Titration of glucose-depleted synaptosomes with pyruvate in the presence of either veratridine or uncoupler stimulates respiration in a Ca²⁺-independent manner. Additionally, the ability of exogenous pyruvate to support the mitochondrial membrane potential in situ is independent of Ca²⁺. It is concluded that Ca²⁺ does not regulate pyruvate oxidation in intact synaptosomes.

【 授权许可】

Unknown