期刊论文详细信息
FEBS Letters | |
Ribosomal localization of the mRNA in the 30S initiation complex as revealed by UV crosslinking | |
Gualerzi, Claudio O.1  Brandt, Roland2  | |
[1] Laboratory of Genetics, Department of Biology, University of Camerino, I-62032 Camerino, Italy;Max-Planck-Institut für Molekulare Genetik, W-1000 Berlin, Germany | |
关键词: Translation initiation; Initiation complex; Ribosome; mRNA; UV-crosslinking; | |
DOI : 10.1016/0014-5793(92)81101-Q | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Translation initiation complexes consisting of 30S ribosomal subunits, 32P-labelled mRNA (002 mRNA), fMet-tRNA and the three initiation factors were subjected to UV-crosslinking to determine the protein and rRNA neighbors of the bound mRNA by immunochemical methods and by nucleic acid hybridization techniques. The mRNA was found to be crosslinked to a specific region of the 16S rRNA spanning from nucleotide 418 to 615 and to ribosomal proteins S1 and S21 (the main targets), S3, S10, S12 and S14; a low level of crosslinking was also detected with S2, S7, S13, S18 and S19.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201912020296991ZK.pdf | 277KB | download |