Ribosomal localization of the mRNA in the 30S initiation complex as revealed by UV crosslinking

FEBS Letters

Gualerzi, Claudio O.¹  Brandt, Roland² 

[1] Laboratory of Genetics, Department of Biology, University of Camerino, I-62032 Camerino, Italy;Max-Planck-Institut für Molekulare Genetik, W-1000 Berlin, Germany

关键词: Translation initiation;    Initiation complex;    Ribosome;    mRNA;    UV-crosslinking;   

DOI  :  10.1016/0014-5793(92)81101-Q

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Translation initiation complexes consisting of 30S ribosomal subunits, ³²P-labelled mRNA (002 mRNA), fMet-tRNA and the three initiation factors were subjected to UV-crosslinking to determine the protein and rRNA neighbors of the bound mRNA by immunochemical methods and by nucleic acid hybridization techniques. The mRNA was found to be crosslinked to a specific region of the 16S rRNA spanning from nucleotide 418 to 615 and to ribosomal proteins S1 and S21 (the main targets), S3, S10, S12 and S14; a low level of crosslinking was also detected with S2, S7, S13, S18 and S19.

【 授权许可】

Unknown