Involvement of DNA polymerase β in proliferation of rat liver induced by lead nitrate or partial hepatectomy

FEBS Letters

Libonati, Massimo³  Columbano, Amedeo³  Ogura, Tsutomu²  Menegazzi, Marta¹  Esumi, Hiroyasu²  Suzuki, Hisanori¹  de Prati, Alessandra Carcereri¹  Ledda-Columbano, Giovanna Maria³ 

[1] Istituto di Chimica Biologica, Università di Verona, 37134 Verona, Italy;National Cancer Center Research Institute, Tokyo, Japan;Istituto di Farmacologia e Patologia Biochimica, Università di Cagliari, 09100 Cagliari, Italy

关键词: DNA polymerase;    β;    Regenerating liver;    Lead nitrate;   

DOI  :  10.1016/0014-5793(92)81314-C

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have studied the expression pattern of DNA polymerase β in two different models of in vivo cell proliferation. Both mRNA levels and enzyme activity of DNA polymerase β markedly increased before and/or during DNA synthesis in proliferating hepatocytes in mitogen-treated and partially hepatectomized rats. The time—courses of the expression of the gene coding for DNA polymerase β were significantly different in the two cell systems. A 5-fold increase in DNA polymerase β mRNA was observed 8 h after lead nitrate administration, i.e. well before the onset of DNA synthesis. In the regenerative liver cells a 3-fold increase in the amount of mRNA was observed 24–48 h after partial hepatectomy, the event being coincident with extensive DNA synthesis. In both systems, the increase of mRNA levels was always paralleled by an increase in enzyme activity, suggesting that DNA polymerase β activity may be regulated at a pre-translational level.

【 授权许可】

Unknown