Activation of a novel form of phospholipase A2 during liver regeneration

FEBS Letters

Nemenoff, Raphael A.¹  Van Putten, Vicki¹  Wojtaszek, Paul A.¹ 

[1] Department of Medicine, University of Colorado School of Medicine, Health Sciences Center, Box C281, 4200 East 9th Avenue, Denver, CO 80262, USA

关键词: Phospholipase A2;    Regenerating liver;    Arachidonic acid;    PLA2;    phospholipase A2;    PC;    phosphatidylcholine;    PE;    phosphatidylethanolamine;    HEPES;    4-(2-hydroxyethyl)-l-piperazineethanesulfonic acid;    EGTA;    [ethylenebis(oxyethylenenitrilo)]tetraacetic acid;   

DOI  :  10.1016/0014-5793(95)00556-O

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Activation of phospholipase A₂ (PLA₂) occurs following mitogenic stimulation of cells. This study examined PLA₂ activation during liver regeneration. Increased activity was detected within 1 h after partial hepatectomy, was maximal by 6 h, and returned to control levels by 24 h. Fractionation of cell-free extracts revealed multiple peaks of PLA₂ activity. One peak appeared identical to the previously described cPLA₂, and was modestly stimulated during regeneration. A higher molecular weight form (hPLA₂) was stimulated ∼5-fold during regeneration. This enzyme was Ca ²⁺-dependent and selective for arachidonoylphosphatidylethanolamine. The activation of this novel form of PLA₂ represents an early event in liver regeneration, and is likely to contribute to the proliferative response.

【 授权许可】

Unknown