Fcε receptor mediated Ca2+ influx into mast cells is modulated by the concentration of cytosolic free Ca2+ ions

FEBS Letters

Dar, Ora¹  Pecht, Israel¹ 

[1]Department of Chemical Immunology, The Weizmann Institute of Science, Rehovot 76100, Israel

关键词: Mast cell;    Rat basophilic leukemia cell (RBL-2H3);    Type 1 Fcε receptor;    Ca2+ influx;    Signal transduction;    Thapsigargin;    [Ca2+]i;    cytosolic free Ca2+ ion concentration;    EGTA;    ethylene glycol diamine N;    N;    N′N′-tetraacetic acid;    FcεRI;    Fcε-receptor;    type 1;    FCS;    fetal calf serum;    InsP3;    inositol 1;    4;    5-trisphosphate;    InsP4;    inositol 1;    3;    4;    5-tetrakisphosphate;    RBL-2H3;    rat basophilic leukemia cells;    subline 2H3;    TG;    thapsigargin;   

DOI  :  10.1016/0014-5793(92)81311-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The relationship between the Fcε receptor mediated stimulation of mast cells and the Ca²⁺ signal it induces were studied using thapsigargin (TG), a blocker of the endoplasmic reticulum Ca²⁺ pump. TG induced, in mucosal mast cells (RBL-2H3 line), a dose-dependent and an InsP₃-independent increase in [Ca²⁺]_i (from resting levels of 83–150 nM to 600–680 nM), and a secretory response amounting to 30–50% of that observed upon FcεRI clustering. The TG induced rise of [Ca²⁺]_i is most probably provided by both arrest of its uptake by the endoplasmic reticulum and influx from the medium. Thus, Ca²⁺ influx in mast cells may be modulated by the [Ca²⁺]_i level.

【 授权许可】

Unknown