Molecular cloning and expression of the human melanocyte stimulating hormone receptor cDNA

FEBS Letters

Chhajlani, Vijay¹  Wikberg, Jarl E.S.¹ 

[1] Department of Pharmacology, Umeå University, Sweden

关键词: Melanocyte stimulating hormone;    Receptor;    cDNA;    Expression;   

DOI  :  10.1016/0014-5793(92)80820-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Melanocytes and melanoma cells are known to possess receptors for melanocyte stimulating hormone (MSH). A cDNA clone, designated IID, has been isolated from human melanoma cells and encodes a MSH receptor. The cloned cDNA encodes a 317 amino acid protein with transmembrane topography characteristics of a G-protein-coupled receptor, but it does not show striking similarity to already published sequences of other G-protein-coupled receptors. When IID cDNA is expressed in COS-7 cells, it binds an ¹²⁵I-labelled MSH analogue (NDP-MSH) in a specific manner. The bound ligand could be displaced by melanotropic peptides, α-MSH, β-MSH, γ-MSH and ACTH (adrenocorticotropic hormone), but not by the non-melanotropic peptide, β-endorphine. This is the first report of the cloning of a receptor gene of the melanotropin receptor family.

【 授权许可】

Unknown