cDNA cloning and characterization of A3i, an alternatively spliced rat A3 adenosine receptor variant

FEBS Letters

Domingo, Ron C.¹  Boyle, David L.¹  Sajjadi, Fereydoun G.¹  Firestein, Gary S.¹ 

[1] Gensia Inc., 9360 Towne Centre Drive, San Diego, CA 92121, USA

关键词: A3;    A3i;    Receptor;    Adenosine;    Rat;    cDNA;    I-ABA;    N 6-(4-amino-3-iodobenzyladenosine;    DPCPX;    1;    3-dipropyl-8-cyclopentylxanthine;    XAC;    xanthine amine congener;    Ado;    denosine;    kb;    kilo base pairs;    PCR;    polymerasechain reaction;    RT-PCR;    reverse transcriptase PCR;   

DOI  :  10.1016/0014-5793(96)00150-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A cDNA encoding a variant form of the A3 adenosine (Ado) receptor was isolated from rat by reverse transcription of brain mRNA followed by PCR. The full-length receptor (A3i) cDNA encodes 337 amino acids and shares complete sequence identity with the rat A3 Ado receptor, except for the presence of a seventeen amino acid insert located in the second intracellular domain. In contrast to the rat A3 receptor, stable expression of A3i in CHO cells resulted in poor coupling to Gl proteins Analysis of receptor transcripts by RT-PCR suggests that the A3 Ado receptor mRNAs are products of alternative splicing. Sequence analysis of A3 genomic DNA Identified a 1.7 kb intron that is likely alternatively spliced to produce the A3 and A3i receptors.

【 授权许可】

Unknown