Endothelial nitric oxide synthase is myristylated

FEBS Letters

Förstermann, Ulrich¹  Klinghofer, Vered¹  Pollock, Jennifer S.¹  Murad, Ferid¹ 

[1] Vascular Biology Group, Abbott Laboratories, Abbott Park, IL 60064, USA

关键词: Myristic acid;    Palmitic acid;    EDRF;    l-Arginine;    Bovine aortic endothelial cell;   

DOI  :  10.1016/0014-5793(92)80816-Y

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The enzyme responsible for the synthesis of endothelium-derived relaxing factor and/or nitric oxide in the endothelium has been described as a particulate enzyme, whereas other isoforms of nitric oxide synthase are soluble enzymes. Here we are reporting that endothelial cells metabolically incorporate myristate (C14), but not palmitate (C16), into nitric oxide synthase. We are postulating that the endothelial-derived nitric oxide synthase is a particulate enzyme because of the fatty acid acylation of the protein which ‘anchors’ the enzyme into the membrane either directly or via another membrane-bound protein.

【 授权许可】

Unknown