A selective inhibitor of cyclic AMP‐dependent protein kinase, N‐[2‐bromocinnamyl(amino)ethyl]‐5‐isoquinolinesulfonamide (H‐89), inhibits phosphatidylcholine biosynthesis in HeLa cells

FEBS Letters

Geilen, Christoph C.¹  Reutter, Werner¹  Wieder, Thomas¹  Wieprecht, Marcus¹ 

[1] Institut für Molekularbiologie und Biochemie, Freien Universität Berlin, Arnimallee 22, D-1000 Berlin 33 (Dahlem), Germany

关键词: Phosphatidylcholine biosynthesis;    cAMP-dependent protein kinase;    N-[2-Bromocinnamyl(amino)ethyl]-5-isoquinolinesulfonamide (M-89);    Forskolin;    HeLa cell;    PC;    phosphatidylcholine;    H-89;    N-[2-bromocinnamyl(amino)ethyl]-5-isoquinolinesulfonamide;    TPA;    12-tetradecanoyl-phorbol-13-acetate;    PBS;    phosphate-buffered saline;   

DOI  :  10.1016/0014-5793(92)80811-T

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In this study, we report that the potent and selective inhibitor of cyclic AMP-dependent protein kinase, N-[2-bromocinnamyl(amino)ethyl]-5-isoquinolinesulfonamide (H-89) interferes with the incorporation of choline into phosphatidylcholine in HeLa cells. Treatment of cells with 10 μM H-89 for 1 h decreases the phosphatidylcholine biosynthesis by 50%. This inhibition is prevented by simultaneous addition of 10 μM forskolin, while the choline uptake itself is not affected by H-89.

【 授权许可】

Unknown