Artifactual detection of ADP‐dependent sucrose synthase in crude plant extracts

FEBS Letters

Perata, P.¹  Yamaguchi, J.¹  Pozueta-Romero, J.¹  Akazawa, T.¹ 

[1] Research Institute for Biochemical Regulation, School of Agricultural Sciences, Nagoya University, Chikusa, Nagoya 404-01, Japan

关键词: Nucleoside diphosphate kinase;    Sucrose synthase;    Invertase;    ADPG;    ADP-glucose;    NDPK;    nucleoside diphosphate kinase;    SS;    sucrose synthase;    G6PDH;    glucose 6-P dehydrogenase;    PGI;    P-glucoisomerase;   

DOI  :  10.1016/0014-5793(92)80790-N

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Results presented in a previous report from this laboratory indicated the presence, in crude extracts from sycamore (Acer pseudoplatanus) and spinach (Spinacea oleracea), of a sucrose synthase (EC 2.4.1.13) showing high affinity for ADP as the glucose acceptor in the sucrose-cleaving reaction. In the present paper we report that the modified enzymatic method previously used to measure sucrose synthase activities leads to the detection of artifactual ADP-dependent sucrose synthase, which in fact arises from the combined action of invertase (EC 3.2.1.26) and nucleoside diphosphate kinase (EC 2.7.4.6) activities. We also present data on the partial purification of nucleoside diphosphate kinase from sycamore cells.

【 授权许可】

Unknown