Comparative analysis of mitochondrial and amyloplast adenylate translocators

FEBS Letters

Pozueta-Romero, Javier¹  Viale, A.M.¹  Akazawa, T.¹ 

[1] Research Institute for Biochemical Regulation. School of Agriculture. Nagova University. Chikusa, Nagoya 464-01, Japan

关键词: ADPglucose: Amyloplast;    Mitochodrion: Silicone oil layer filtering centrifugation;    ADPG;    ADPglucose;    At;    atractyloside;    BKA;    bongkrekic acid;    CAT;    carboxyatractyloside;   

DOI  :  10.1016/0014-5793(91)80016-V

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Structurally intact and metabolically competent mitochondria isolated from liquid-culture cells of sycamore (Acer pseudoplatanus L.) were shown to incorporate ADPglucose. Employing the double silicone oil layer filtering centrifugation method, we examined the kinetic properties of the uptake of various adenylates as well as the inhibitory effects exerted by carboxyatractyloside, atractyloside and bongkrekic acid, known specific inhibitors of the mitochondrial adenylate translocator. Immunoblot patterns of peptides derived from the partial proteolytic digestion of the mitochondrial and plastid adenylate translocators were shown to be essentially the same. We conclude that the molecular entities engaged in the adenylate transport system operating in two different organelles. mitochondria and amyloplasts, are very similar.

【 授权许可】

Unknown