The effect of dilauroyl‐l‐3‐phosphatidylcholine on the interaction between cytochrome P‐450 1A1 and benzo[a]pyrene

FEBS Letters

Friedman, Fred K.¹  Omata, Yoshiaki¹ 

[1] Laboratory of Molecular Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA

关键词: Cytochrome P-450;    Dilauroyl-l-3-phosphatidylcholine;    Benzo[a]pyrene;   

DOI  :  10.1016/0014-5793(92)80782-C

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Fluorescence quenching of benzo[a]pyrene (BP) by cytochrome P-450 1A1 was used to probe the effect of the lipid, dilauroyl-l-3-phophatidylcholine, on this substrate-enzyme interaction. In the presence of lipid, a monoclonal antibody to this P-450 maximally inhibited BP binding at an antibody-to-P-450 ratio of 1:2, corresponding to an antibody crosslinked P-450 complex. The antibody did not inhibit BP binding in the absence of lipid. These results indicate that when P-450 is subjected to the orientational constraints imposed by antibody-mediated crosslinking, the lipid alters the conformation or quanternary structure of the P-450 oligomer in a manner which changes its affinity for BP.

【 授权许可】

Unknown