Synthesis of the proline analogue [2,3‐3H]azetidine‐2‐carboxylic acid Uptake and incorporation in Arabidopsis thaliana and Escherichia coli

FEBS Letters

Verbruggen, Nathalie¹  van Montagu, Marc¹  Messens, Eric¹ 

[1] Laboratorium voor Genetica, Universiteit Gent, B-9000 Gent, Belgium

关键词: Amino acid;    4-Aminobutyric acid;    Escherichia coli;    Synthesis of radioactive compounds;    GABA;    γ-amino-butyric acid a 4-amino-butyric acid;    2AZ;    azetidine-2-carboxylic acid;    PPO;    2;    5-diphenyloxazole;    FLPC;    fast-protein liquid chromatography;    TCA;    trichloroacetic acid;    TLC;    thin-layer chromatography;   

DOI  :  10.1016/0014-5793(92)81288-W

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Azetidine-2-carboxylic acid, the 4-membered ring noranalogue or proline, is regularly used in the study or proline metabolism as well as the study of protein conformation. We prepared ??-[2,3-³H]azetidine-2-carboxylic acid with an optimized 10% yield from commercially available 4-amino-[2,3-³H]butyric acid. Purification was performed by fast-protein liquid chromatography. The biological activity was checked in both Arabidopsis thaliana and Escherichia coli. The obtained specific activity or 10 mCl/mmol was sufficient for most uptake and incorporation studies.

【 授权许可】

Unknown