Human immunodeficiency virus type 1 productive infection in staurosporine‐blocked quiescent cells

FEBS Letters

Aranda-Anzaldo, A.¹  Viza, D.¹ 

[1] URA-CNRS, Laboratoire d'Immumobiologie, Facultè de Mèdecine, 45, rue des Saints-Pères, 75006 Paris, France

关键词: Protein kinase inhibitor;    Viral infectivity;    Cel cycle;    T cell;    DMSO;    dimethyl sulfoxide;    HIV-1;    human immunodeficiency virus type 1;    IF;    immunofluorescence;    Mab;    monoclonal anti-body;    PBS;    phosphate-buffered saline;    p.i;    post-infection;    TCM;    tissue culture medium;   

DOI  :  10.1016/0014-5793(92)81268-Q

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Staurosporine, an antibiotic known to inhibit cellular protein kinases, can reversibly block the progress of normal and tumour cells into the cell cycle. The ability of HIV-1 to infect and replicate in cells blocked by staurosporine was investigated. The results show that blocked, non-cycling cells can be productively infected by HIV-1, steadily releasing infectious progeny virus for several weeks. This suggests that at least in some cases, HIV-1 can be found in a stable and active state in resting, non-proliferating T cells.

【 授权许可】

Unknown