Class II tubulin, the major brain β tubulin isotype is polyglutamylated on glutamic acid residue 435

FEBS Letters

Weber, Klaus²  Klöppel, Klaus-Dieter¹  Plessman, Uwe²  Rüdiger, Manfred²  Wehland, Jürgen¹ 

[1] Gesellschaft für Biotechnologische Forschung, Mascheroder Weg 1, 3300 Braunschweig, Germany;Max Planck Institute for Biophysical Chemistry, Department of Biochemistry, Postfach 2841, 3400 Göttingen, Germany

关键词: Glutamylation;    Mass spectrometry;    Posttranslational modification;    Subtilisin;    Tubulin;   

DOI  :  10.1016/0014-5793(92)81061-P

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Protein sequencing shows that porcine brain tubulin retains the N-terminal sequences of α and β tubulin after a mild treatment with subtilisin, C-terminal peptides released by subtilisin were purified and characterized by automated Edman degradation and mass spectrometry. We confirm the polyglutamylation of α tubulin on glutamic acid residue 445 reported by others and show in addition that class 11 β tubulin, the major β tubulin isotype of adult brain, is also polyglutamylated. The substitution is restricted to glutamic acid residue 435. Thus all major tubulin isotypes of adult brain are subjected to polyglutamylation.

【 授权许可】

Unknown