Cell density‐dependent expression of heparin‐binding growth‐associated molecule (HB‐GAM, p18) and its down‐regulation by fibroblast growth factors

FEBS Letters

Merenmies, Jussi¹ 

[1] Institute of Biotechnology and Neurobiology Research Unit, University of Helsinki, Karvaamokuja 3, 00380 Helsinki, Finland

关键词: Heparin-binding;    Cell differentiation;    BALB/c 3T3 cell;    Contact inhibition;    Growth factor;    aFGF;    acidic fibroblast growth factor;    bFGF;    basic fibroblast growth factor;    DMEM;    Dulbecco's modified Eagle's medium;    FCS;    fetal calf serum;    GAPDH;    gylceraldehyde-3-phosphate-dehydrogenase;    HB-GAM;    heparin-binding;    growth-associated molecule;    SDS;    sodium dodecyl sulfate;    TGFβ;    transforming growth factor beta;   

DOI  :  10.1016/0014-5793(92)80699-H

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Heparin-binding growth-associated molecule (HB-GAM) is a developmentally regulated protein that is intensely expressed during the rapid postnatal growth phase of rat brain. The expression of HB-GAM studied in 12 cell lines was restricted to C6 rat glioma cell line and BALB/c 3T3 cells. In BALB/c 3T3 cells the expression of HB-GAM enhanced in confluent and quiescent cell cultures. When the confluent cultures were treated with bFGF the expression of HB-GAM mRNA was strongly reduced and the protein disappeared rapidly from proliferating cells. The data presented suggest involvement of HB-GAM in cell differentiation phenomena rather than in cell proliferation.

【 授权许可】

Unknown