Spectroscopic identification of the haem ligands of cellobiose oxidase

FEBS Letters

Jones, Gareth D²  Thomson, Andrew J¹  Cox, Mark C¹  Moore, Geoffrey R¹  Wilson, Michael T²  Rogers, Melanie S²  Cheesman, Myles¹ 

[1] Centre for Metalloprotein Spectroscopy and Biology, School of Chemical Sciences, University of East Anglia, Norwich NR4 7TJ, UK;Department of Chemistry and Biological Chemistry, University of Essex, Wivenhoe Park, Colchester C04 3SQ, UK

关键词: Cellobiose oxidase;    Flavocytochrome b;    Electron paramagnetic resonance;    Nuclear magnetic resonance;    Magnetic circular dichroism;    D2O/HOD;    deuterium oxide/deuterium hydroxide ϵ;    extinction coefficient;    EPR;    electron paramagnetic resonance;    FAD;    flavin adenine dinucleotide;    His;    histidine;    MCD;    magnetic circular dichroism;    Met;    methionine;    NMR;    nuclear magnetic resonance;   

DOI  :  10.1016/0014-5793(92)80774-B

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A spectroscopic study of the flavocytochrome b enzyme, cellobiose oxidase, employing optical, NMR, EPR and near infra-red MCD techniques, has identified the axial ligands of the b-type haem. These are a histidine and a methionine, and this ligation set is discussed in relation to the functional role of the haem group.

【 授权许可】

Unknown