Cellobiose oxidase of Phanerochaete chrysosporium enhances crystalline cellulose degradation by cellulases

FEBS Letters

Renganathan, V.¹  Bao, Wenjun¹ 

[1] Department of Chemical and Biological Sciences, Oregon Graduate Institute of Science and Technology, 19600 N.W. von Neumann Drive, Beaverton, OR 97006-1999, USA

关键词: Cellobiose oxidase;    Crystalline cellulose;    Cellulose degradation;    Cellulase;    Phanerochaete chrysosporium;    Trichoderma viride;    CBO;    cellobiose oxidase;    FPLC;    fast protein liquid chromatography;   

DOI  :  10.1016/0014-5793(92)80289-S

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect of Phanerochuete chrysosporium cellobiose oxidase (CBO) on microcrystalline cellulose hydrolysis by Trichoderma cellulases was determined. Addition of 10 μg ml⁻¹ CBO to a reaction mixture containing T. viride cellulase increased glucose and cellobiose production by 10% and 48%, respectively. Cellulose weight loss was also enhanced by 19%. Al higher concentrations (20–80 μg ml⁻¹). CBO decreased glucose and cellobiose production. Cellulose weight loss at 60 μ ml⁻¹ CBO was 76% compared to control cellulase reactions. This decrease appears to be due to inactivation of cellulase by H₂O₂ produced via CBO reaction, because addition of catalase enhances sugar production and cellulose weight loss. These findings suggest that at low, perhaps physiologically relevant concentrations, CBO enhances crystalline cellulose degradation by cellulases.

【 授权许可】

Unknown