Tetrahydro‐6‐biopterin is associated with tetrahydro‐7‐biopterin in primary murine mast cells

FEBS Letters

Hültner, Lothar¹  Ziegler, Irmgard² 

[1] GSF-Institut für Experimentelle Hämatologie, Marchioninistr.25, D-8000 München 70, Germany;GSF-Institut für Klinische Molekularbiologie und Tumorgenetik, Marchioninistr.25, D-8000 München 70, Germany

关键词: Mast cell;    Tetrahydro-6-biopterin;    Tetrahydro-7-biopterin;    6-Biopterin;    7-Biopterin;    Tryptophan 5-mono-oxygenase;    H4-6- or 7-biopterin;    6- or 7-(l-erythro-1′;    2′-dihydroxy-propyl)-5;    6;    7;    8-tetrahydropterin;    6- or 7-biopterin;    6- or 7-(l-erythro-1′;    2′-dihydroxypropyl)-pterin;    6- or 7-neopterin;    6- or 7-(l-erythro-1′;    2′;    3′-trihydroxypropyl)-pterin;    monapterin;    6-(l-threo-1′;    2′;    3′-trihydroxypropyl)-pterin;    6-pyruvoyl-H4pterin;    6-pyruvoyl-5;    6;    7;    8-tetrahydropterin;    IL-3;    interleukin 3;   

DOI  :  10.1016/0014-5793(92)80755-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Murine bone marrow-derived mast cells proliferate in response to interleukin 3. In addition to 6-biopterin, 7-biopterin was identified in these cells by HPLC analysis of iodine oxidized extracts and by alkaline permanganate oxidation to the 6- and 7-carboxylic acids. 7-Biopterin comprised 31.9 (± 7.7)% of the total biopterin. It was absent in cells which were grown with of l-p-chlorophenylalanine, an inhibitor of tryptophan 5-monooxygenase. Both 6- and 7-biopterin were present in the cell as their tetrahydro forms. From these data we conclude that 7-biopterin, in contrast to e.g. brain tissue, regularly occurs as a normal metabolite in primary mast cells and that it is generated during hydroxylation of tryptophan.

【 授权许可】

Unknown