Construction and functional analysis of hybrid interleukin‐6 variants Characterization of the role of the C‐terminus for species specificity

FEBS Letters

Leebeek, Frank W.G.¹  Fowlkes, Dana M.¹ 

[1] University of North Carolina at Chapel Hill, Department of Phatology, Chapel Hill, NC, USA

关键词: Interleukin-6;    Interleukin-6 receptor;    Hybrid protein;    Species specificity;    Growth factor;   

DOI  :  10.1016/0014-5793(92)81013-C

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have constructed several hybrid human interleukin-6 (IL-6) variants in which the carboxyl-terminus, which includes a receptor binding site of IL-6 has been replaced with the C-terminus of various proteins homologous to human IL-6, IL-6 hybrids with the C-terminus of human growth hormone and human granulocyte-colony stimulating factor maintain part of the biological activity of human IL-6. Replacing the C-terminus of human IL-6 with the C-terminus of mouse and rat IL-6 resulted in a normal or increased activity on a mouse cell line; however, this gave a low (to 200-fold less) activity on a human cell line compared to wild-type human IL-6. We therefore conclude that the C-terminus of IL-6 plays in important role in the species specificity of IL-6.

【 授权许可】

Unknown