期刊论文详细信息
FEBS Letters
Deduced amino acid sequence and E1–E2 equilibrium of the sarcoplasmic reticulum Ca2+‐ATPase of frog skeletal muscle Comparison with the Ca2+‐ATPase of rabbit fast twitch muscle
Andersen, Jens Peter1  Vilsen, Bente1 
[1] Danish Biomembrane Research Centre, Institute of Physiology, University of Aarhus, DK-8000 Aarhus C, Denmark
关键词: Ca2+-ATPase;    Sarcoplasmic reticulum;    Amino acid sequence comparison;    Frog muscle;    Ca2+ affinity;    bp;    base pair(s);    Ca2+-ATPase;    Ca2+-activated adenosine triphosphatase;    EGTA;    [ethylenebis(oxyethylene-nitrilo)]-tetraacetic acid;    ER;    endoplasmic reticulum;    kb;    kilo bases;    MOPS;    3-(N-morpholino)propanesulfonic acid;    oligo(dT);    oligodeoxythymidine;    poly(A+);    polyadenosine;    TES;    N-tris[-hydroxymethyl]methyl-2-aminoethanesulfonic acid;    SDS;    sodium dodecyl sulfate;    SERCA;    sarco(endo)plasmic reticulum Ca2+-ATPase;    SR;    sarcoplasmic reticulum;   
DOI  :  10.1016/0014-5793(92)81003-5
学科分类:生物化学/生物物理
来源: John Wiley & Sons Ltd.
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【 摘 要 】

The cDNA encoding a Ca2+-transport ATPase of frog (Rana esculenia) skeletal muscle was isolated and characterized. The deduced amino acid sequence, consisting of 994 residues, showed 89% identity to the fast twitch muscle sarcoplasmic reticulum Ca2+-ATPases of chicken and rabbit. Northern blot analysis using a fragment of this cDNA as probe detected a 5.0 kb message in frog skeletal muscle but did not detect any mRNA encoding sarcoplasmic reticulum Ca2+-ATPase in frog cardiac muscle. The enzymatic properties of the amphibian skeletal muscle Ca2+-ATPase were compared with those of the rabbit fast twitch muscle Ca2+-ATPase by functional expression of the cDNAs in COS-1 cells. The amphibian Ca2+-ATPase displayed a reduced apparent affinity for Ca2+ and an increased apparent affinity for the inhibitors, vanadate and thapsigargin, relative to the mammalian enzyme. This may be explained by a mechanism in which relatively more of the E2 conformation accumulated 1n the frog Ca2+-ATPase than in the mammalian enzyme.

【 授权许可】

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