Expression of c‐fos, c‐jun, jun‐B, metallothionein and metalloproteinase genes in human chondrocyte

FEBS Letters

Cloutier, Jean-Marie¹  Pelletier, Jean-Pierre³  Zafarullah, Muhammad³  Martel-Pelletier, Johanne³  Gedamu, Lashitew² 

[1] Department of Surgery, Hôpital Saint-Luc, 1058 St-Denis St., Montréal, Qué., H2X 3J4, Canada;Department of Biological Sciences, University of Calgary, 2500 University Drive N.W., Calgary, T2N 1N4 Alta., Canada;Rheumatic Disease Unit, Notre-Dame Hospital Research Centre, Department of Medicine, University of Montreal, 1560 Sherbrooke St. East, Montréal, Qué, Canada

关键词: Osteoarthritis;    Chondrocyte;    fos/jun;    Collagenase;    Stromelysin;    Metallothionein;    mRNA;    Ap-1;    activating protein-1;    hMT-IIA;    human metallothionein IIA;    OA;    osteoarthritic;   

DOI  :  10.1016/0014-5793(92)80992-P

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Normal and osteoarthritic (OA) human articular cartilage chondrocytes, released enzymatically in the presence of 0.5% fetal calf serum, display constitutive expression of early response activating protein (AP-1) genes; c-fos, c-^jun and jun-B. Among the late AP-1 responsive genes, total matallothionein (MT) and stromelysin mRNAs were expressed at high levels in both normal and OA chondrocytes, while collagenase and hMT-II_A mRNA levels were elevated only in OA individuals. Despite the common AP-1 sequences present in their promoter regions, the three late genes were differentially expressed.

【 授权许可】

Unknown