Detection of VAC‐β (annexin‐8) in human placenta

FEBS Letters

Hauptmann, Rudolf²  Pepinsky, R.Blake¹ 

[1] Department of Protein Chemistry, Biogen, Inc., 14 Cambridge Center, Cambridge MA 02142, USA;Ernst Boehringer Institut für Arzneimittelforschung, Fa. Bender Co, Dr. Boehringergasse 5-11, A1121 Vienna, Austria

关键词: Annexin-8;    VAC-β;    Lipocortin;    Calcium/phospholipid binding protein;    VAC;    vascular anticoagulant;    DEAE;    diethylaminoethyl;    CNBr;    cyanogen bromide;    DSS;    disuccinimidyl suberate;    PLBP;    calcium-dependent phospholipid binding protein;   

DOI  :  10.1016/0014-5793(92)80843-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A36 kDa calcium/phospholipid binding protein in human placenta was identified as VAC-β (annexin-8) by a combination of immunological and peptide mapping analyses. The protein is a minor product in placenta, accounting for less than 1% of extracted annexins. From 150 g of tissue, only 100 μg of the protein was isolated. By anion-exchange chromatography on diethylaminoethyl-cellulose annexin-8 coeluted with annexin-3. By gel filtration, the protein chromatographed as a broad peak, where half the product eluted as a monomer and half eluted as a heterodimer that was associated with a 10 kDa subunit. The combination of annexin-8 being a minor component in standard annexin preparations and it co-eluting with annexin-3 by ion exchange chromatography are likely to account for the failure of other labs to characterize the product.

【 授权许可】

Unknown