The nucleotide sequence of the S RNA of Impatiens necrotic spot virus, a novel tospovirus

FEBS Letters

de Haan, Peter¹  Gielen, Jan J.L.²  Goldbach, Rob¹  Peters, Dick¹  Westerbroek, Annemarie²  de Avila, Antonio C.¹  Kormelink, Richard¹ 

[1] Department of Virology, Agricultural University Wageningen, Binnenhaven 11, PO Box 8045, 6700 EM Wageningen, The Netherlands;Zaadunle B.V., Department of Biotechnology, Westeinde 62, PO Box 26, 1600 AA Enkhuizen, The Netherlands

关键词: Impatiens necrotic spot virus;    cDNA cloning;    S RNA sequence;    Ambisense gene arrangement;    Nucleocapsid protein;    Non-structural protein;   

DOI  :  10.1016/0014-5793(92)80830-A

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Impatiens necrotic spot virus (INSV) shares a number of properties with tomato spotted wilt virus (TSWV), the type species of the genus tospovirus within the family Bunyaviridae. INSV, however, differs from TSWV in plant host range and serology. In order to define the genomic structure and the taxonomic status of this TSWV-Iike virus, the nucleotide sequence of its genomic S RNA segment has been determined. The molecular data obtained demonstrate that, like TSWV, INSV has an ambisense S RNA molecule, encoding a non-structural protein in viral sense and the nucleocapsid protein in viral complementary sense. The level of nucleotide sequence homology between their S RNAs, as well as the divergence in amino acid sequence homology of their gene products, confirm previous conclusions from serological studies that INSV and TSWV represent distinct virus species within the newly created genus, tospovirus.

【 授权许可】

Unknown