Isolation and characterization of a human cDNA clone encoding a novel DNA topoisomerase II homologue from HeLa cells

FEBS Letters

Austin, Caroline A.¹  Fisher, L.Mark¹ 

[1] Molecular Genetics Group, Department of Cellular and Molecular Sciences, St George's Hospital Medical School, University of London, London, UK

关键词: DNA topoisomerase II;    Human cell line;    cDNA cloning;    Isoenzyme;    Protein sequence homology;   

DOI  :  10.1016/0014-5793(90)81520-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have isolated and sequenced 3 human DNA topoisomerase II (topo II) partial cDNA clones from a HeLa carcinoma cell cDNA library. Two clones were identical to an internal fragment of HeLa topo II cDNA. The third clone, CAA5, had a different and novel sequence which shared significant nucleotide (62%) and predicted peptide (70%) homologies with a region of the HeLa topo II cDNA. Our results suggest that HeLa cells express at least two homologous forms of DNA topoisomerase II. The new HeLa topo II homologue is discussed in relation to topo II isoenzymes recently described in a Burkitt lymphoma and other cell lines.

【 授权许可】

Unknown