期刊论文详细信息
FEBS Letters | |
Stereospecific assignments and χ1 rotamers for FKBP when bound to ascomycin from 3 J Hα,Hβ and 3 J N,Hβ coupling constants | |
Fesik, Stephen W.1  Olejniczak, Edward T.1  Xu, Robert X.1  | |
[1] Pharmaceutical Discovery Division, Abbott Laboratories, Abbott Park, IL 60064, USA | |
关键词: FKBP; Ascomycin; Coupling constant; NMR; NOE; nuclear Overhauser effect; FKBP; FK506 binding protein; 3D HSQC-TOCSY; three-dimensional heteronuclear single quantum correlation-total correlation spectroscopy; 3D HCCH-TOCSY; three-dimensional 1H-13C-13C-1H correlation via 13C total correlation spectroscopy; E.COSY; exclusive correlation spectroscopy; | |
DOI : 10.1016/0014-5793(92)80881-G | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
3 J Hα,Hβ and 3 J N,Hβ coupling constants were measured for isotopically labeled FKBP when bound to the immunosuppressant, ascomycin, using a 1H-coupled 3D HCCH-TOCSY and 15N-coupled 3D HSQC-TOCSY experiment, respectively. From an analysis of these two sets of coupling constants, stereospecific β-proton assignments and χ1 rotamers for FKBP have been obtained. All of the χ1 rotamers were consistent with the χ1 angles measured in the X-ray crystal structure of the FKBP/FK506 complex, suggesting that the structures of the two complexes are similar.
【 授权许可】
Unknown
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