Cloning and expression of a glycine transporter from mouse brain

FEBS Letters

Nelson, Nathan¹  López-Corcuera, Beatriz¹  Liu, Qing-Rong¹  Nelson, Hannah¹  Mandiyan, Sreekala¹ 

[1] Roche Institute of Molecular Biology, Roche Research Center, Nutley, NJ 07110, USA

关键词: Neurotransmitter;    Transport;    Glycine;    Cloning;    Expression;   

DOI  :  10.1016/0014-5793(92)80875-H

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have isolated a cDNA clone from a mouse brain library encoding the glycine transporter (GLYT). Xenopus oocytes injected with a synthetic mRNA accumulated [³H]glycine to levels of up to 80-fold above control values. The uptake was specific for glycine and dependent on the presence of Na⁺ and Cl⁻ in the medium. The cDNA sequence predicts a highly hydrophobic protein of 633 amino acids with 12 potential transmembrane helices. The predicted amino acid sequence has 40–45% identity to the GABA, noradrenaline, serotonin and dopamine transporters. This implies that all of these neurotransmitter, transporters may have evolved from a common ancestral gene that diverged into the GABA, glycine and catecholamine subfamilies at nearly the same time.

【 授权许可】

Unknown