Electrochemical Assay of Neurotransmitter Glycine in Brain Cells

Bulletin of the Korean chemical society

Suw Young Ly¹  Dong Young Kim¹ 

关键词: Voltammetry;    Neurotransmitter;    Glycine;    Fish;    Brain;   

DOI  :  

学科分类：化学（综合）

来源: Korean Chemical Society

【 摘 要 】

Neurotransmitter glycine in the nano gram range was analyzed using a paste electrode (PE) in cyclic voltammetry (CV) and square-wave stripping voltammetry (SWSV). An anodic peak caused by oxidation of the glycine ion appeared at the 0.4 V (versus Ag/AgCl/KCl) potential in a 0.1 M NH₄H₂PO₄ electrolyte solution. At optimized conditions, the working range of the SWSV and CV concentration was found to be 5-60 ngL^-1 glycine; precision of R² = 0.9816 (SWSV) and 0.9986 (CV); and detection limit of 0.65 ngL^-1 (5.82 �? 10^-12 molL^-1) (S/N = 3). The optimized conditions were applied to an assay in a fish brain tissue and a living brain cell in real time.

【 授权许可】

Unknown