期刊论文详细信息
FEBS Letters
Heat‐labile enterotoxin crystal forms with variable A/B5 orientation Analysis of conformational flexibility
Sixma, Titia K.1  Kalk, Kor H.1  Wartna, Ellen S.1  van Scheltinga, Anke C.Terwisscha1  Hol, Wim G.J.1  Aguirre, Angel1 
[1] BIOSON Research Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
关键词: Heat-labile enterotoxin;    Toxin;    Conformational change;    LT;    heat-labile enteroioxin;    rms;    root mean square;    ccf;    correlation coefficient;    PC;    Patterson correlation;   
DOI  :  10.1016/0014-5793(92)80869-I
学科分类:生物化学/生物物理
来源: John Wiley & Sons Ltd.
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【 摘 要 】

A new native crystal form of heat-labile enterotoxin (LT) has two AB5 complexes in the asymmetric unit with different orientations of the A subunit with respect to the B pentamer. Comparison with other crystal forms of LT shows that there is considerable conformational freedom for orientating the A subunit with respect to the B pentamer. The rotations of A in different crystal forms do not follow one specific axis, but most of them share a hinge point, close to the main interaction area between A and B5. Analysis of the two high-resolution structures available shows that these rotations cause very little change in the actual interactions between A and B5.

【 授权许可】

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