Heat‐labile enterotoxin crystal forms with variable A/B5 orientation Analysis of conformational flexibility

FEBS Letters

Sixma, Titia K.¹  Kalk, Kor H.¹  Wartna, Ellen S.¹  van Scheltinga, Anke C.Terwisscha¹  Hol, Wim G.J.¹  Aguirre, Angel¹ 

[1] BIOSON Research Institute, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands

关键词: Heat-labile enterotoxin;    Toxin;    Conformational change;    LT;    heat-labile enteroioxin;    rms;    root mean square;    ccf;    correlation coefficient;    PC;    Patterson correlation;   

DOI  :  10.1016/0014-5793(92)80869-I

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A new native crystal form of heat-labile enterotoxin (LT) has two AB₅ complexes in the asymmetric unit with different orientations of the A subunit with respect to the B pentamer. Comparison with other crystal forms of LT shows that there is considerable conformational freedom for orientating the A subunit with respect to the B pentamer. The rotations of A in different crystal forms do not follow one specific axis, but most of them share a hinge point, close to the main interaction area between A and B₅. Analysis of the two high-resolution structures available shows that these rotations cause very little change in the actual interactions between A and B₅.

【 授权许可】

Unknown