Transcripts of the viroid central conserved region contain the local tertiary structural element found in full‐length viroid

FEBS Letters

Robertson, H.D.¹  Paul, C.P.¹  Levine, B.J.¹  Branch, A.D.² 

[1] Department of Biochemistry, Cornell University Medical College, New, York, NY 10021, USA;Center for Studies of the Biological Correlates of Addiction, The Rockefeller University, New York, NY 10021, USA

关键词: Viroid;    RNA structure;    UV-crosslinking;    Viroid-host interaction;   

DOI  :  10.1016/0014-5793(92)80644-V

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The viroid central conserved region (CCR) is highly conserved among different viroids and is thought to be involved in viroid replication. A novel tertiary structure occurs in the CCR of native circular potato spindle tuber RNAs. To permit more detailed studies of this structural element, a small RNA oligonucleotide containing the CCR of the viroid genome was synthesized. The tertiary structure of these CCR transcripts was examined by UV-crosslinking of the RNA, followed by mapping of the crosslink using limited alkaline digestion and classical RNA secondary analysis. The CCR transcript was found to undergo UV-crosslinking between the same two bases as in full-length viroid, indicating that the tertiary structure is the same and that the CCR transcript will be useful for the affinity purification of host components.

【 授权许可】

Unknown