Yeast ADP/ATP carrier (AAC) proteins exhibit similar enzymatic properties but their deletion produces different phenotypes

FEBS Letters

Gavurniková, G.²  Kolarov, J.²  Drgon̆, T.¹  S̆abová, L.¹ 

[1] Cancer Research Institute, Slovak Academy of Sciences, 812 32 Bratislava, Czechoslovakia;Department of Biochemistry, Komensky University, 842 15 Bratislava, Czechoslovakia

关键词: ADP/ATP transport;    Mitochondria;    AAC gene;    Saccharomyces cerevisiae;   

DOI  :  10.1016/0014-5793(92)80637-V

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Saccharomyces cerevisiae strains expressing a single type of ADP/ATP carrier (AAC) protein were prepared from a mutant in which all AAC genes were disrupted, by transformation with plasmids containing a chosen AAC gene. As demonstrated by measurements of [¹⁴C]ADP specific binding and transport, all three translocator proteins, AAC1, AAC2 and AAC3 when present in the mitochondrial membrane, exhibited similar translocation properties. The disruption of some AAC genes, however, resulted in phenotypes indicating that the function of these proteins in whole cells can be quite different. Specifically, we found that the disruption of AAC1 gene, but not AAC2 and AAC3, resulted in a change in colony phenotype.

【 授权许可】

Unknown