YGE1 is a yeast homologue of Escherichia coli grpE and is required for maintenance of mitochondrial functions

FEBS Letters

Toh-e, Akio¹  Uno, Isao²  Yoshida, Satoshi¹  Ikeda, Eri¹  Mitsuzawa, Hiroshi³ 

[1] Department of Biology, Faculty of Science, The University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113, Japan;Research and Development Laboratories 1, Nippon Steel Corporation, 1618, Ida, Kawasaki 211, Japan;Department of Microbiology and Molecular Genetics, University of California, Los Angeles, CA 90024, USA

关键词: Mitochondria;    grpE;    Saccharomyces cerevisiae;    Essential gene;    DAPI;    4';    6'-diamidino-2-phenylindole;    DiOC6;    3;    3'-dihexyloxacarbocyanine iodide;    ORF;    open reading frame;    PKC;    protein kinase C;    PCR;    polymerase chain reaction;    YPD;    yeast extract-peptondextrose;    SD;    synthetic minimal medium;   

DOI  :  10.1016/0014-5793(94)80428-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The grpE gene is a heat shock gene of Escherichia coli whose product functions as a chaperone to (re)fold proteins. We found a yeast homologue of grpE and designated it YGE1. YGE1 can replace grpE in E coli, indicating that YGE1 is a functional homologue of grpE. Deletion of YGE1 is lethal. During depletion of the Ygel product, mitochondria are sequestered in mother cells thereby accumulating cells without mitochondria, suggesting that Ygel protein plays a pivotal role in maintaining mitochondrial functions.

【 授权许可】

Unknown